Mechanism of Yanghe Decoction against subcutaneous tumor in pulmonary metastasis from breast cancer through HIF-1α signaling pathway regulating glycolysis:based on network pharmacology and animal experiment / 中国中药杂志

This study aims to explore the mechanism of Yanghe Decoction(YHD) against subcutaneous tumor in pulmonary metastasis from breast cancer, which is expected to lay a basis for the treatment of breast carcinoma with YHD. The chemical components of medicinals in YHD, and the targets of the components were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and SwissTargetPrediction. The disease-related targets were searched from GeneCards and Online Mendelian Inheritance in Man(OMIM). Excel was employed to screen the common targets and plot the Venn diagram. The protein-protein interaction network was constructed. R language was used for Gene Ontology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment. A total of 53 female SPF Bablc/6 mice were randomized into normal group(same volume of normal saline, ig), model group(same volume of normal saline, ig), and low-dose and high-dose YHD groups(YHD, ig, 30 days), with 8 mice in normal group and 15 mice in each of the other groups. Body weight and tumor size was measured every day. Curves for body weight variation and growth of tumor in situ were plotted. In the end, the subcutaneous tumor sample was collected and observed based on hematoxylin and eosin(HE) staining. The mRNA and protein levels of hypoxia inducible factor-1α(HIF-1α), pyruvate kinase M2(PKM2), lactate dehydrogenase A(LDHA), and glucose transporter type 1(GLUT1) were detected by PCR and Western blot. A total of 213 active components of YHD and 185 targets against the disease were screened out. The hypothesis that YHD may regulate glycolysis through HIF-1α signaling pathway to intervene in breast cancer was proposed. Animal experiment confirmed that the mRNA and protein levels of HIF-1α, PKM2, LDHA, and GLUT1 in the high-and low-dose YHD groups were lower than those in the model group. YHD has certain inhibitory effect on subcutaneous tumor in pulmonary metastasis from breast cancer in the early stage, which may intervene pulmonary metastasis from breast cancer by regulating glycolysis through HIF-1α signaling pathway.

Effect of Modified Chaihu Guizhi Ganjiang Tang in Treating Chronic Cholecystitis of Gallbladder Heat / 中国实验方剂学杂志

Objective: To explore the clinical effect of modified Chaihu Guizhi Ganjiang Tang in the treatment of chronic cholecystitis with gallbladder heat and spleen cold syndrome. Method: Totally 129 cases with chronic cholecystitis treated in our hospital from March 2017 to July 2018 were selected as study objectives and randomly divided into observation group (65 cases)and control group (64 cases). After the shedding and exclusion cases were subtracted, 61 patients in observation group and 61 patients in control group actually completed this study. The routine treatment was provided to both groups, and modified Chaihu Guizhi Ganjiang Tang was additionally given to observation group. All of the cases received treatments for 3 weeks. The two groups' clinical effect, traditional Chinese medicine(TCM)syndrome scores, inflammatory factors[interleukin (IL)-6, tumor necrosis factor (TNF)-α] levels in serum, gallbladder function, gastrointestinal function indicators[motilin (MTL), vasoactive intestinal peptide (VIP)] before and after treatments, as well as toxic side reaction were compared. Result: Totally 7 cases were lost during the study period. The overall effective rate of the observation group was 95.08%(58/61), which was significantly higher than 80.33%(49/61)of the control group (Pα concentrations after treatment were significantly lower than those before treatment (PPPConclusion: Modified Chaihu Guizhi Ganjiang Tang treats patients with chronic cholecystitis with bile heat and spleen cold syndrome by effectively relieving inflammatory response and accelerating the recovery of gastrointestinal function and gallbladder systolic function, with an exact curative effect and high patient tolerance.